Binding of Deoxyribonucleic Acid-Dependent Deoxyribonucleic Acid Polymerase to Poxvirus
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چکیده
منابع مشابه
Deoxyribonucleic acid-dependent ribonucleic acid polymerase of Caulobacter crescentus.
Deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) was purified from the dimorphic bacterium Caulobacter crescentus at three stages in development. Enzyme from pure populations of stalked cells, as well as populations enriched in swarmer and predivisional cells, appeared identical in subunit structure and template requirements. The molecular weights of the enzyme sub...
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Echinomycin is a peptide antibiotic which binds strongly to double-helical DNA up to a limit of approximately one molecule per five base-pairs. There is no detectable interaction with rRNA and only extremely feeble non-specific interaction with poly(rA)-poly(rU). Heat denaturation of DNA greatly decreases the binding, and similarly limited interaction is observed with naturally occurring single...
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DNA polymerase III has been purified 4,500-fold from the Escherichia coli mutant, HMS83, which lacks DNA polymerases I and II. When subjected to disc gel electrophoresis, the most purified fraction exhibits a single major protein band from which enzymatic activity may be recovered. Polyacrylamide gel electrophoresis under denaturing conditions produces two protein bands with molecular weights o...
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Aphidicolin, a known inhibitor of eucaryotic deoxyribonucleic acid (DNA) polymerase alpha, efficiently inhibited amplification of ribosomal DNA during oogenesis in Xenopus laevis. DNA polymerase alpha, but not DNA polymerase gamma, as isolated from ovaries, was sensitive to aphidicolin. DNA polymerase beta was not detectable in Xenopus ovary extracts. Therefore, DNA polymerase alpha plays a maj...
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DNA polymerase catalyzes the formation of a phosphodiester bond between the 3’-hydroxyl terminus of a DNA chain and the cu-phosphate of a deoxyribonucleoside 5’-triphosphate (2). There is a single binding site in the Escherichia co& enzyme for which all four common deoxyribonucleoside triphosphates compete (3). A wide variety of analogues of the deoxyribonucleoside triphosphates can also bind t...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1972
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.9.1.70-74.1972